Str Typing from Bloodstained Fabric without Dna Extraction
نویسندگان
چکیده
Whole blood from various individuals was spotted on 100% white cotton fabric. Once the bloodstains were dry, approximately 1 mm or less of the bloodstained thread was cut from each sample and placed in an autoclaved reaction tube containing either TE buffer or sterile distilled water. The tubes were then subjected to a simple preincubation period as described by the author previously (1). After the preincubation was completed, a mixture of respective PCR Reaction Buffer, Primers and AmpliTaq Gold Polymerase was added into each of the tubes. The bloodstained threads now submerged in the mixture were then amplified.
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